CCRG-03. STUDY OF PIWI-INTERACTING RNAS IN GLIOBLASTOMA PATHOLOGY: A NEW LEVEL OF REGULATION OF GLIOBLASTOMA STEM CELLS?

Abstract BACKGROUND One of the putative mechanisms early recurrence GBM is existence glioblastoma stem cells (GSCs) that can resist therapy and subsequently establish new foci. PIWI-interacting RNAs (piRNAs), responsible for maintaining genome stability, may then play a key role in their biology, particularly germ cells. A number piRNA molecules are also promising biomarkers useful prognosis prediction disease progression.Material METHODS Glioblastoma were cultured DMEM/F12 medium supplemented with bFGF EGF growth factors. DMEM containing 10% fetal bovine serum (FBS) was used to culture differentiated RNA isolated from selected paired primocultures checked quality by capillary electrophoresis. NEBNext Small Library Prep Set preparation cDNA libraries. Sequencing analysis performed using Next500/550 High Output v2 Kit 75 cycles NextSeq500 sequencer. RESULTS Twenty primary cultures prepared verify expression CD133 SOX2 GSCs, ability tumors immunodeficient mice, differentiate. At this time, we finished sequencing acquired data bioinformatically analysed. Subsequently, will perform piRNAs be diagnostic prognostic on samples non-tumor brain controls. All results presented at conference. CONCLUSION Based recent studies, it evident have significant impact biology. Their within GSCs provide tool identifying targeting these could usable as biomarker patients.The study supported programme project Ministry Health Czech Republic reg. no. NV19-03-00501, NV19-03-00559, Conceptual development research organization FNBr, 65269705